BIOLOGICAL SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES AND THEIR FORMULATION

Abstract

Nanotechnology is a rapidly developing medical technology with several applications in the pharmaceutical sciences. The goal of the current study is to create a formulation that contains nanoparticles of silver and investigates their capability against specific fungal infections. Emblica Phyllanthus leaf was used for the preparation of nanoparticles and characterization of nanoparticles was done. It was seen that size of AgNPs was 27nm they were spherical in shape and no contamination was found in the extract. Before the preparation of formulation, the toxicity of AgNps was checked on the mice model, and the safest concentration of nanoparticles was selected. For the synthesis of the final concentration oil and water emulsion was prepared and AgNPs of 5% concentration were mixed. The disc diffusion method was used to check in vitro antifungal activity of AgNps and the nanoparticles were found to be effective against Candida, Aspergillus and Trichophyton. For invivo study, approval was taken from the University’s ethical committee and Ayub Hospital and written consent was taken from the patients. Patients suffering from fungal infections such as tinea Mannum, tinea versicular, tinea capitis, and tinea faciei. were selected and the formulation was topically applied on the skin of the volunteers. Among the infections tine versicolar was the most common and prevalent in men ages (21-40). As a result, formation showed excellent antifungal effect 100% recovery was seen in patients and no harmful effect was seen.